Cytochalasin B inhibition of endothelial proliferation at wound edges in vitro.

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Cytochalasin B inhibition of endothelial proliferation at wound edges in vitro

Cytochalasin B prevents both migration and wound-associated replication when applied to wounded monolayers of bovine endothelium in tissue culture. The normal low background rate of replication in undisturbed areas of the cultures is not inhibited by cytochalasin B. These results suggest that some form of movement may be required for initiation of wound-associated replication by endothelial cells.

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Most re-endothelialization requires both cell migration and cell proliferation. To study the association between cell migration and the initiation of DNA synthesis in an in vitro wound model, confluent cultures of porcine aortic endothelial cells grown on glass coverslips were scraped to remove half of the monolayer. Treatment with 1 ng/ml of taxol, a microtubule stabilizing drug, for 24 hours ...

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ژورنال

عنوان ژورنال: Journal of Cell Biology

سال: 1979

ISSN: 0021-9525,1540-8140

DOI: 10.1083/jcb.81.2.348